MIST Linker™ enables rapid, site-specific conjugation of primary antibodies with a biotin-tagged oligonucleotide DNA through a UV-cleavable linker. The kit provides a fast, flexible solution for generating antibody–DNA conjugates for a wide range of applications, which may include:

• Multiplex IF/IHC and cyclic imaging

• Spatial proteomics

• DNA-barcoded antibody assays

• Single-cell multiomics (e.g., CITE-seq)

• Immuno-PCR and DNA-based signal amplification

• Proximity Ligation Assay (PLA) & Proximity Extension Assay (PEA)

• Custom DNA-functionalized antibody development

• Targeted Therapeutics and Drug Delivery

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The unique features of this kit include

1. Lowest input of antibody as little as 1 µg

• Generate antibody–DNA conjugates in two simple steps using only 1 µg of antibody, dramatically reducing reagent consumption and the cost of building large antibody panels for multiplex assays.

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2. UV-cleavable DNA conjugation

• Antibody-bound DNA can be rapidly released using standard UV illumination. The released DNA can serve as a barcode, template for signal amplification, or molecular identifier for downstream applications.

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3. Fc-directed conjugation preserves antibody performance

• Site-specific conjugation to the antibody Fc region minimizes interference with antigen binding and helps maintain antibody affinity.

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4. Streamlined purification

• An optimized purification workflow efficiently removes excess oligonucleotides, unreacted MIST Linker™, and other small molecules, yielding high-quality conjugates ready for use.

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5. Plug-and-play oligonucleotide conjugation

• Simply pair any biotinylated oligonucleotide with the MIST Linker™ to rapidly functionalize virtually any compatible antibody—eliminating the need for custom oligonucleotide conjugation chemistry.

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6. Ready in under one hour

• Complete the entire antibody–DNA conjugation workflow in less than one hour, enabling on-demand preparation whenever experiments require.