MIST Linker™ enables rapid, site-specific labeling of primary antibodies with a fluorophores through a UV-cleavable linker. The kit provides a fast, flexible solution for labeling antibodies for a typical immunofluorescence staining or immunohistochemistry, with comparable performance as conventional 2-step process. The labeled antibody can carry up to 8 fluorophores, and thereafter it overcomes the major limitation of using labeled primary antibody for staining.​

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The unique features of this kit include

1. Lowest input of antibody as little as 0.5 µg

• Generate dye labeled antibodies in one simple steps using only 0.5 µg of antibody, dramatically reducing reagent consumption and the cost of building large antibody panels for multiplex assays.

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2. High fluorophore load

• Labeling of up to 8 fluorophores on each antibody leading to super high fluorescence using just primary antibody for staining.

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3. UV cleavage for removal of fluorescence

• The fluorophores on an antibody can be rapidly released using standard UV illumination. This feature makes MIST Linker particularly suitable for multiplex staining. 

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4. Fc-directed labeling preserves antibody performance

• Site-specific conjugation to the antibody Fc region minimizes interference with antigen binding and helps maintain antibody affinity.

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5. Ideal for multiplex and multi-cycle staining

• Proven technique for multiplex assays. Cleavable design for cyclic staining. No need to worry about antibody sources and same species in the same panel.

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6. Ready in 5 - 10 min

• Complete the entire antibody labeling workflow in 5 - 10 min, enabling on-demand preparation whenever experiments require.