MIST Linker enables rapid plug-in/plug-out labeling of antibodies
TM
MIST Linker is the first and only reagent that rapidly links primary, unlabeled antibodies with fluorophores or biomolecules such as oligonucleotide DNAs, while the label can be easily removed. This uniqe plug-in/plug-out feature provides enormous advantages for many applications beyond immunostaining. Additionally, MIST Linker significantly prevents the waste of expensive antibodies and shortens conventional staining workflows.
TM
Broad antibody compatibility
Photocleavable for multiplex imaging
Small amount of input antibody as low as 0.5 μg
Fast labeling of as short as 5 min
Simple conjugation with purification
Flexible linking of antibodies with molecules
Easy conjugation; easy dissociation
Custom antibody–DNA conjugation on demand. Simply pair any biotinylated oligonucleotide with virtually any antibody in minutes using as little as 1 µg of antibody—no lengthy conjugation protocols or large antibody quantities required. UV-cleavable chemistry enables rapid deconjugation, allowing DNA to be removed from antibodies whenever needed.
FAQ & Knowledge tips:
1. MIST Linker works for antibody solutions containing BSA, tris buffer, and preservatives.
Labeling with fluorophores or conjugation with DNAs is not affected by the presence of BSA, tris buffer, glycerol and preservatives.
2. MIST Linker permits multiplexing of antibodies even from the same species.
When the labeled antibodies are mixed for multiplexed assays, the unreacted linkers are already removed by MIST quencher so they don't interfere with the assay.
3. The labeled fluorophore or DNA can be cleaved and released from stained cells/tissue.
Common handheld UV lamp is good enough to photocleave the labeled molecules on antibodies. The released DNA can be collected for specific downstream assays such as PCR. Typically >90% labeled molecules can be released.
4. Conjugated antibodies are pure without small contaminants through additional filtration.
Additional purification with 100 KDa ultracentrifugation filter can remove the majority of small molecules.
5. Labeling of antibodies can be done within 5-10 min and one step while longer time could be slightly better.
It is not a concern for high expression proteins. Two steps for conjugating DNA to antibodies with longer time can overcome steric hindrance and often results in higher efficiency of labeling.
6. Any DNA or RNA can be easily conjugated to antibodies.
Any DNA/RNA with biotin tagged can be conjugated with antibodies with MIST Linker immediately. We save the cost for the users as biotin ended DNA/RNA is relatively inexpensive, while the prevailing crosslinking methods by chemicals consume large amount of antibodies and cost much more.
7. As low as 0.5 µg primary antibody is sufficient for labeling. No waste of expensive antibodies in the lab.
Most antibodies in the market are not exactly as they are labeled. For example, labeled 0. 5 mg/mL could be only 0.3 mg/mL. Each MIST Linker kit and protocol offer much more excess to ensure always saturation of labeling.
8. Adaption to antibodies of low concentration.
Some vendors offer antibodies in very low concentration. Use MIST quencher to remove excess MIST Linker. If not for multiplex IF, may not need to remove the excess reagent.