Simpler, but more powerful than
flow and imaging cytometry
Cutting-edge technology
Our single-cell omics microdevices are developed with the latest cutting-edge technology coupled with advanced algorithms and visualization programs to ensure maximum throughput, accuracy and efficiency and deliver reliable results.
Portable, affordable all-in-one kit
Only a common fluorescence microscope or imaging instrument is needed. No training is needed. Our portable, disposable devices can be operated anywhere and for most cell samples and tissue specimens. More importantly, it is affordable and accessible!
Quality Assurance
At MIST Bioscience, we are committed to providing the highest quality products to our customers. We have strict quality assurance protocols in place to ensure that our diagnostic devices and biological reagents meet the highest industry standards.
Expert Customer Support
Our team of experts is always available to provide support and answer any questions you may have about our products. We pride ourselves on providing exceptional customer service and support.
Reasons to choose cytoMIST technology
01.
Rapid assay - same duration as IHC
02.
Single-cell resolution - Large area of tissue or cell culture but no damage
03.
High throughput -
>20 samples/day
04.
High multiplexity - ~20-50 markers at a time; possibly up to 450
05.
No special instrument - common microscope or scanner enough
Work flow
Multiplex in situ tagging (MIST) array is based on barcode microbeads to quantitate functional proteins and transcripts through fluorescence reading or sequencing. It copies the in situ biological information from tissue or plated cells.
Immunostaining of your sample
Overlay MIST device with sample & UV exposure
10 min
Microscopy imaging or scanning
Visualization & analysis with MIST-Explorer
Knowledge tips:
1. cytoMIST can assay precious cells of small sample size.
Our technology can process a cell sample as small as 1,000 cells. It works for both suspension cells and adherent cells as well as thin tissue sections, as long as the sample is on a flat surface. It doesn't work for 3D culture.
2. cytoMIST doesn't need access of special, expensive instruments.
A common fluorescence microscope that has scanning function of large image is sufficient. The microscope should be able to image bright field and two fluorescence channels. We provide a suite of software to stitch images, data extraction and analysis.
3. cytoMIST is born for high-content analysis.
It typically assays 6-30 markers at a time, whereas many high-multiplex methods only image 3-4 colors a time with a few cycles. Thus, cytoMIST shows more advantages with higher multiplexity.
4. Various sample sources such as FFPE samples from mouse and human are applicable.
As long as your samples can be processed by IHC or IF, they can be analyzed by cytoMIST too.
5. Users can freely built their marker panel powered by MISTlinker.
Scientists often want to analyze the proteins of their own interest. To our best knowledge, no vendor can satisfy such a demand yet. We build a library of hundreds of protein targets and have strictly validated them. You can simply select the targets and compose your own assay panels. The reagents of the custom panel will be shipped to you.
Spatial proteomic service
Send samples to us for single-cell spatial proteomic analysis. Our team will deliver a detailed report, including both raw and processed data, for your review and further insights. You can also use other bioinformatic tools for further analysis.
Step 1
Discussion with our expert to understand your sample and biological questions.
Step 2
Preliminary tests on your samples, generation of custom panel and evaluation of feasibility.
Step 3
Spatial proteomic measurement and data analysis. Results in report and visualization in MIST-Explorer.
Sample requirement
Cultured cells
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Cell lines: Adherent cells cultured in a 12 or 6 well plate, with confluency 30 - 80%.
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Primary cells: same above. Must ensure monolayer. Do not form clumps or 3D structure.
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Other culture substrates: Adhesive slides that provides excellent cell adhesion.
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Suspension cells need to be fixed on a flat, transparent surface, or in single-cell microwells.
Tissue sections
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Sectioning at 3 - 5 μm thick. No more than 3 sections / glass slide.
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FFPE and fresh frozen sample sections are acceptable.
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Adhesive slides are preferred that provides excellent tissue adhesion.
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Lay completely flat, no wrinkles, no bubbles, uniform thickness on glass slide.
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Only 3mm x 3mm or 5mm x 5mm sub-region detected for large samples.